14-3-3 epsilon Rabbit Polyclonal Antibody

• Catalog Number: orb6357
• Category: Antibodies
• Description: 14-3-3 epsilon Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 14-3-3E (151-255/255aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: YWHAE
• UniProt ID: P62258
• RRID: AB_10938720
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 14-3-3 epsilon; 14 3 3 E; 14 3 3 epsilon; 14 3 3E;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

A novel Pyrin-Associated Autoinflammation with Neutrophilic Dermatosis mutation further defines 14-3-3 binding of pyrin and distinction to Familial Mediterranean Fever Ann Rheum Dis, 76, 2085-2094 (2017)

PubMed: 28835462

Applications

WB

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (orb6357) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (14-3-3 epsilon) Polyclonal Antibody, Unconjugated (orb6357) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A431 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-14-3-3 epsilon (orb6357) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kD, Observed band size: 29 kD.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Placenta tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Rat Placenta tissue lysates, Lane 6: Human 293T cell lysates, Lane 7: Human A375 cell lysates, Lane 8: Human SW480 cell lysates, Primary: Anti-14-3-3 epsilon (orb6357) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29 kDa, Observed band size: 30 kDa.