14-3-3 antibodyCustom conjugation service available

Catalog Number: orb624373

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CategoryPrimary antibodies
Catalog Numberorb624373
Immunogentyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC-P, IP, WB
Dilution rangeWB: 1:1000-1:10000, IP: 1:200-1:2000, IHC-P: 1:20-1:200, IF: 1:20-1:200
Form/AppearanceLiquid: PBS with 0.02% sodium azide and 50% glycerol pH 7.3
ReactivityHuman, Mouse, Rat
ConjugationUnconjugated
UniProt IDP27348
MW28 kDa
IsotypeIgG
Target14-3-3
StorageStore at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
DescriptionRabbit polyclonal antibody to 14-3-3
Expiration Date12 months from date of receipt.
NoteFor research use only.
Purity>95% as determined by SDS-PAGE, Immunogen affinity purified
  1. 10 images
    • Immunofluorescence image of pig kidney tissue using anti- 14-3-3 zeta/delta (dilution at 2.5 ug/ml)
    • Immunohistochemical staining of paraffin embedded human thyroid cancer tissue using  14-3-3 zeta/delta antibody (primary antibody at 2.5 ug/ml)
    • Western blot analysis of mouse spleen (lane 1), mouse uterus (lane 2), rat large intestines (lane 3), human thyroid cancer (lane 4), human breast cancer (lane 5), Hela cells (slane 6) using 14-3-3 zeta/delta antibody (1 ug/ml)
    • IF analysis of pig kidney tissue using anti- 14-3-3 zeta/delta (dilution of primary antibody at 2.5 ug/ml)
    • IHC-P staining of pig kidney tissue using  14-3-3 zeta/delta antibody (dilution at 2.5 ug/ml)
    • Immunohistochemical staining of paraffin embedded pig spleen tissue using anti- 14-3-3 zeta/delta (primary antibody at 2.5 ug/ml)
    • IHC-P image of rat lung tissue using  14-3-3 zeta/delta antibody (dilution of primary antibody at 2.5 ug/ml)
    • IHC-P staining of pig uterus tissue using anti- 14-3-3 zeta/delta (dilution at 2.5 ug/ml)
    • Immunohistochemical staining of paraffin embedded pig kidney tissue using  14-3-3 zeta/delta antibody (primary antibody at 2.5 ug/ml)
    • Immunohistochemical staining of paraffin embedded pig uterus tissue using anti- 14-3-3 zeta/delta (primary antibody at 2.5 ug/ml)
    14-3-3 zeta/delta antibody [orb382906] Custom conjugation service available

    ICC, IF, IHC-P, WB

    Human, Mouse, Porcine, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 200 μg
  2. 8 images
    • Western blot analysis of EC109(Lane 1), 293T(Lane 2) whole cell lysate using 14-3-3 protein beta/alpha antibody
    • Immunohistochemical staining of human small intestine tissue using 14-3-3 protein beta/alpha antibody
    • Western blot analysis of Hela whole cell lysate(lane 1), HepG2 whole cell lysate(lane 2), Raji whole cell lysate(lane 3), 293T whole cell lysate(lane 4), Jurkat whole cell lysate(lane 5), HT29 whole cell lysate(lane 6), Rat brain tissue(lane 7) using 14-3
    • Immunofluorescence analysis of HepG2 cells using 14-3-3 protein beta/alpha antibody
    • Immunohistochemical staining of human testis tissue using 14-3-3 protein beta/alpha antibody
    • Immunohistochemical staining of human kidney tissue using 14-3-3 protein beta/alpha antibody
    • Western blot analysis of Jurkat whole cell lysate(lane 1), HepG2 whole cell lysate(lane 2) using 14-3-3 protein beta/alpha antibody
    • Western blot analysis of Hela whole cell lysate(lane 1), Raji whole cell lysate(lane 2), Mouse liver tissue(lane 3), Mouse brain tissue(lane 4), Rat liver tissue(lane 5), Rat lung tissue(lane 6), Mouse lung tissue(lane 7), Zebrafish lysate(lane 8) using 1
    14-3-3 protein beta/alpha antibody [orb241438]

    ELISA, IF, WB, IHC

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    100 μg, 50 μg
  3. 6 images
    • Immunohistochemistry of paraffin-embedded human colon cancer tissue using YWHAE antibody
    • Western blot aanalysis of A375 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, Mouse brain tissueusing YWHAE antibody
    • Western blot aanalysis of Hela whole cell lysate, A375 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, Mouse heart tissueusing YWHAE antibody
    • Immunofluorescence staining of HepG2 cells using YWHAE antibody
    • Immunohistochemistry of paraffin-embedded human adrenal gland tissue using YWHAE antibody
    • Immunohistochemistry of paraffin-embedded human brain tissue using YWHAE antibody
    YWHAE antibody [orb350816]

    ELISA, IF, WB, IHC

    Human, Mouse

    Rabbit

    Polyclonal

    50 μg, 100 μg
  4. 6 images
    • Immunohistochemistry of paraffin-embedded human brain tissue using 14-3-3 protein eta antibody
    • Western blot aanalysis of Mouse spleen tissueusing 14-3-3 protein eta antibody
    • Western blot aanalysis of HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissueusing 14-3-3 protein eta antibody
    • Immunofluorescence staining of Hela cells using 14-3-3 protein eta antibody
    • Immunohistochemistry of paraffin-embedded human prostate tissue using 14-3-3 protein eta antibody
    • Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using 14-3-3 protein eta antibody
    14-3-3 protein eta antibody [orb241455]

    ELISA, IF, WB, IHC

    Human, Mouse

    Rabbit

    Polyclonal

    100 μg, 50 μg
  5. 5 images
    • Immunohistochemical staining of paraffin embedded human colon carcinoma tissue using YWHAG antibody (primary antibody dilution at: 1:50-100)
    • Western blot analysis of in mouse cerebellum tissue lysates (35ug/lane)using YWHAG antibody (antibody dilution at 1:1000)
    • Western blot analysis of 293 cell lysateusing YWHAG antibody (primary antibody dilution at: 1:1000)
    • Western blot analysis of Hela cell line using YWHAG antibody (dilution of primary antibody at 1:1000)
    YWHAG antibody [orb34357]

    FC, IHC-P, WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    400 μl, 80 μl