tdTomato antibody

• Catalog Number: orb182397
• Category: Antibodies
• Description: Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Purified recombinant peptide produced in E. coli.
• Concentration: 1mg/ml
• Dilution range: WB 1:500-1:2,000 IHC (F) 1:50-1:500 IHC (P) 1:50-1:500 IF 1:50-1:500
• Form/Appearance: ​Polyclonal antibody supplied in PBS, 20% glycerol and 0.05% sodium azide
• Purity: This antibody is epitope-affinity purified from goat antiserum
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: tdTomato
• Protein Sequence: MEQKLISEEDLGTISTMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
• Storage: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: Cherry fluorescent protein, DsRed, mCherry, red fl
• Note: For research use only
• Application notes: In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein)
• Expiration Date: 12 months from date of receipt.

References

Yang, Yang et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency Cell, 169, 243-257.e25 (2017)

PubMed: 28388409

Applications

IF

Reactivity

Mouse

Mao, Xiying et al. Single-Cell RNA Sequencing of hESC-Derived 3D Retinal Organoids Reveals Novel Genes Regulating RPC Commitment in Early Human Retinogenesis Stem Cell Reports, (2019)

PubMed: 31543471

Applications

ICC

Reactivity

Human

Li Li # 1 2 3, Yu He # 1 2 3, Kai Liu 4, Lin Liu 1 2 3, Shan Shan 1 2 3, Helin Liu 1 2 3, Jiangbo Ren 1 2 3, Shujie Sun 1 2 3, Min Wang 1 2 3, Jidong Jia 5 6 7, Ping Wang GITRL impairs hepatocyte repopulation by liver progenitor cells to aggravate inflammation and fibrosis by GITR+CD8+ T lymphocytes in CDE Mice Cell Death Dis, 15, 114 (2024)

PubMed: 38321001

Applications

IF

Reactivity

Mouse

Jia Sun #1, Xinyuan Wang #12, Rui Sun 1, Xiaoao Xiao 1, Yu Wang 1, Yu Peng 1, Yuanqing Gao 3 Microglia shape AgRP neuron postnatal development via regulating perineuronal net plasticity Mol Psychiatry, (2023)

PubMed: 38001338

Applications

IF

Reactivity

Mouse

Jian Ge 1, Hongxia Shao 1 2, Hongxu Ding 3, Yuefeng Huang 4, Xuebing Wu 5, Jie Sun 6, Jianwen Que Single Cell Analysis of Lung Lymphatic Endothelial Cells and Lymphatic Responses during Influenza Infection J Respir Biol Transl Med, 1, 10003 (2024)

PubMed: 38529320

Applications

IF

Reactivity

Mouse

Pomaville, Matthew B. et al. Follicle-innervating A[delta]-low threshold mechanoreceptive neurons form receptive fields through homotypic competition Neural Dev, 18, 2 (2023)

PubMed: 37106422

Applications

IHC-P

Reactivity

Mouse

Fang, Yinshan et al. Epithelial Wntless regulates postnatal alveologenesis Development, 149, dev199505 (2022)

PubMed: 34931663

Applications

IF, IHC

Reactivity

Mouse

Li, Hui et al. Identification of novel B-1 transitional progenitors by B-1 lymphocyte fate-mapping transgenic mouse model Bhlhe41 dTomato-Cre Front Immunol, 13, 946202 (2022)

PubMed: 36189231

Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells Elife, 11, e78217 (2022)

PubMed: 36129169

Miller, Daniel S. et al. Neuronal Dystroglycan regulates postnatal development of CCK/cannabinoid receptor-1 interneurons Neural Dev, 16, 4 (2021)

PubMed: 34362433

SOX9 Modulates the Transformation of Gastric Stem Cells Through Biased Symmetric Cell Division Gastroenterology, S0016-5085(23)00109-9 (2023)

PubMed: 36740200

Therapeutic resistance and susceptibility is shaped by cooperative multi-compartment tumor adaptation Cell Death Differ, 26, 2416-2429 (2019)

PubMed: 30824837

Yartseva, Valeria et al. Heterogeneity of Satellite Cells Implicates DELTA1/NOTCH2 Signaling in Self-Renewal Cell Rep, 30, 1491-1503.e6 (2020)

PubMed: 32023464

Interleukin-9 protects from early podocyte injury and progressive glomerulosclerosis in Adriamycin-induced nephropathy Kidney Int, 98, 615-629 (2020)

PubMed: 32446933

LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis Cell, 178, 1478-1492.e20 (2019)

PubMed: 31474362

Applications

IF, IHC

Jiang, Ming et al. VEGF receptor 2 (KDR) protects airways from mucus metaplasia through a Sox9-dependent pathway Dev Cell, 56, 1646-1660.e5 (2021)

PubMed: 34010630

Hu, Yanyan et al. Induction of mouse totipotent stem cells by a defined chemical cocktail Nature, (2022)

PubMed: 35728625

Zhang, Lianghui et al. Single-cell transcriptomic profiling of lung endothelial cells identifies dynamic inflammatory and regenerative subpopulations JCI Insight, 7, e158079 (2022)

PubMed: 35511435

He, Jianbo et al. DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration NPJ Regen Med, 7, 21 (2022)

PubMed: 35351894

Zhang, Junren et al. Tel2 regulates redifferentiation of bipotential progenitor cells via Hhex during zebrafish liver regeneration Cell Rep, 39, 110596 (2022)

PubMed: 35385752

Gribben, Christopher et al. Ductal Ngn3-expressing progenitors contribute to adult β cell neogenesis in the pancreas Cell Stem Cell, (2021)

PubMed: 34478642

Gu, Bin et al. Live imaging YAP signalling in mouse embryo development Open Biol, 12, 210335 (2022)

PubMed: 35042406

Western blot analysis of HEK293 cell line lysate using tdTomato antibody.

Confocal immunofluoroscence analysis of HEK293 cells using tdTomato antibody.