ZYX monoclonal antibody (M01), clone 2C10-4A7

• Catalog Number: orb2291949
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant ZYX.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: ZYX (AAH08743, 1 a.a. ~ 572 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MAAPRPSPAISVSVSAPAFYAPQEKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYECEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT
• Tested applications: ELISA, IF, IP, WB
• Clone Number: 2C10-4A7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH08743

Detection limit for recombinant GST tagged ZYX is 1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to ZYX on HeLa cell. [antibody concentration 10 ug/ml]

Immunoprecipitation of ZYX transfected lysate using anti-ZYX monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ZYX MaxPab rabbit polyclonal antibody.

Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7. Lane 1: ZYX transfected lysate (61.3 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ZYX monoclonal antibody (M01), clone 2C10-4A7 (Cat # orb2291949). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (88.66 KDa).