ZWINT-1 Antibody

• Catalog Number: orb1529648
• Category: Antibodies
• Description: ZWINT-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IP, WB
• Reactivity: Human
• Immunogen: between 225 and C-term
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; ICC-IF - 1:50 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ZWINT-1
• UniProt ID: O95229
• NCBI: NP_008988.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HZwint-1; ZW10 interactor, kinetochore protein; zw
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ZWINT-1 by WB and IP.Samples: HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-ZWINT-1 antibody used for WB at 0.04 µg/ml (A) and at 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).

Detection of human ZWINT-1 by ICC. Sample: HeLa cells. Antibody: Affinity purified rabbit anti-ZWINT-1 used at a dilution of 1:200. Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated.