ZNF81 Antibody

• Catalog Number: orb1267495
• Category: Antibodies
• Description: ZNF81 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ZNF81 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-197 amino acids from the N-terminal region of human ZNF81.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 76 kDa
• Target: ZNF81
• UniProt ID: P51508
• NCBI: P51508
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Zinc finger protein 81, HFZ20, ZNF81
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of U251 cell stained with ZNF81 Antibody (N-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF81 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZNF81 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.

Western blot analysis in 293 cell line lysates (35 ug/lane).