ZNF622 Antibody / ZPR9

• Catalog Number: orb2636807
• Category: Antibodies
• Description: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF622 (zinc-finger protein 622), also known as ZPR9 (zinc-fingerlike protein 9), is a 477 amino acid protein that localizes to both the nucleus and the cytoplasm. Expressed in liver, spleen, lung, kidney and brain, ZNF622 is thought to activate the bound transcription factor B-Myb and, through this activation, may play a role in embryonic development. ZNF622 contains two U1-type zinc fingers and exists as either a homodimer or a heterodimer that can be phosphorylated by MELK (maternal embryonic leucine zipper kinase). Overexpression of ZNF266 may be associated with liver metastases, carcinomatoses and colorectal carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-ZNF622-1C11
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human ZNF622 protein was used as the immunogen for the ZNF622 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ZNF622 antibody is available for research use only.
• UniProt ID: Q969S3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ZNF622 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using ZNF622 antibody (green, clone PCRP-ZNF622-1C11) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with ZNF622 antibody (blue, clone PCRP-ZNF622-1C11), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZNF622 antibody (clone PCRP-ZNF622-1C11). These results demonstrate the foremost specificity of the PCRP-ZNF622-1C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.