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Catalog Number | orb1525199 |
---|---|
Category | Antibodies |
Description | ZNF518A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | between 1300 and 1350 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | ZNF518A |
UniProt ID | Q6AHZ1 |
NCBI | NP_055618.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | ZNF518; zinc finger protein 518A; zinc finger prot Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human ZNF518A by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ZNF518A antibody orb1525199 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. ZNF518A was more efficiently immunoprecipitated by rabbit anti-ZNF518A antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep |
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