ZNF408 Antibody / PRDM17

• Catalog Number: orb2636817
• Category: Antibodies
• Description: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the Kr ppel C2H2-type zinc-finger protein family, ZNF396 (zinc finger protein 396), also known as PRDM17 (PR domain zinc finger protein 17), is a 720 amino acid nuclear protein that contains ten C2H2-type zinc fingers. The gene encoding ZNF408 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-ZNF408-1E5
• Tested applications: FACS, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human ZNF408 protein was used as the immunogen for the ZNF408 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ZNF408 antibody is available for research use only.
• UniProt ID: Q9H9D4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ZNF408 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

FACS staining of PFA-fixed human HeLa cells with ZNF408 antibody (blue, clone PCRP-ZNF408-1E5), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZNF408 antibody (clone PCRP-ZNF408-1E5). These results demonstrate the foremost specificity of the PCRP-ZNF408-1E5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.