ZNF326 Antibody

• Catalog Number: orb1527624
• Category: Antibodies
• Description: ZNF326 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 532 and 582
• Concentration: 200 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:250 - 1:1,000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ZNF326
• UniProt ID: Q5BKZ1
• NCBI: NP_892021.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: zinc finger protein interacting with mRNPs and DBC
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ZNF326 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF326 antibody orb1527624 used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate. ZNF326 was also immunoprecipitated by rabbit anti-ZNF326 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A) and 3 seconds (B).

Detection of human ZNF326 by immunohistochemistry.Sample: FFPE sections of human ovarian carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti-ZNF326 (Cat. No. orb1527624) used at a dilution of 1: 1, 000 (0.2µg/ml) and 1: 400 (0.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100