ZMYM3 Antibody

• Catalog Number: orb2636803
• Category: Antibodies
• Description: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZMYM3 (zinc finger MYM-type protein 3), also known as ZNF261 (zinc finger protein 261), XFIM, DXS6673E or MYM, is a 1,370 amino acid nuclear protein that contains nine MYM-type zinc fingers. Expressed in a variety of tissues, including heart, muscle and brain, ZMYM3 is thought to function as part of a histone deacetylase-containing complex that contains other proteins, such as HDAC1 and HDAC2, and may play a role in gene silencing through the modification of chromatin structure. Defects in the gene encoding ZMYM3 that lead to chromosomal translocations may be a cause of X-linked mental retardation. Two isoforms of ZMYM3 exist due to alternative splicing events.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-ZMYM3-2F10
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human ZMYM3 protein was used as the immunogen for the ZMYM3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ZMYM3 antibody is available for research use only.
• UniProt ID: Q14202
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ZMYM3 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using ZMYM3 antibody (green, clone PCRP-ZMYM3-2F10) and phalloidin (red).

IHC staining of FFPE human breast carcinoma tissue with ZMYM3 antibody (clone PCRP-ZMYM3-2F10). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FACS staining of PFA-fixed human HeLa cells with ZMYM3 antibody (blue, clone PCRP-ZMYM3-2F10), and unstained cells (red).

FACS staining of PFA-fixed human Raji cells with ZMYM3 antibody (blue, clone PCRP-ZMYM3-2F10), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZMYM3 antibody (clone PCRP-ZMYM3-2F10). These results demonstrate the foremost specificity of the PCRP-ZMYM3-2F10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.