ZIC1/2/3/4/5 antibody

• Catalog Number: orb304667
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZIC1/2/3/4/5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIC1/2/3/4/5. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ZIC1
• Entrez: 22773, 85416, 7545, 7546, 22771, 84107, 7547, 22774
• UniProt ID: Q8N9L1, Q61467, O60481, Q62520, Q15915, Q96T25, P46684, Q62521, O95409
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti ZIC1 antibody, anti ZIC antibody, anti ZNF201
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ZIC1/2/3/4/5 expression in rat brain (A) whole cell lysates. (Predicted band size: 48; 55; 50; 36; 68 kD; Observed band size: 55 kD)

Immunofluorescent analysis of ZIC1/2/3/4/5 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.