Zebrafish Malondialdehyde (MDA) ELISA Kit

SKU: orb2667377

Description

The Zebrafish Malondialdehyde (MDA) ELISA Kit is designed to quantify Malondialdehyde (MDA) in zebrafish serum, plasma and other biological fluids. The kit has a detection range of 31.25-2000 ng/mL and a sensitivity of 9.15 ng/mL.

Research Area

Gastroenterology & Hepatology, Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish MDA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish MDA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish MDA in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Zebrafish
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 31.25-2000 ng/mL
• Sensitivity: 9.15 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Malondialdehyde; MDA

Quick Database Links

Gene Symbol

MDA

Adam Bownik, Barbara Pawlik-Skowrońska Effects of cyanobacterial metabolites and their mixtures on biomarkers of oxidative stress in RTgill-W1 cells Toxicol In Vitro, 112, 106195 (2026)

PubMed: 2667377

Applications

ELISA

Reactivity

Fish