Zebrafish ak2 Antibody (N-term)

• Catalog Number: orb1787893
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Zebrafish ak2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3~39 amino acids from the N-terminal region of Zebrafish ak2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Zebrafish
• Immunogen: 3~39
• UniProt ID: Q1L8L9
• MW: 26616 Da
• Tested applications: FC, IHC, WB
• Dilution range: WB: 1:2000, IHC: 1:25, FC: 1:25
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Adenylate kinase 2, mitochondrial {ECO:0000255|HAM
• Note: For research use only

All lanes: Anti-Zebrafish ak2 Antibody (N-term) at 1:2000 dilution. Lane 1: ZF4 whole cell lysate. Lane 2: Zebrafish lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining Zebrafish ak2 in zebra fish body tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Overlay histogram showing ZF4 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.