ZCCHC8 Antibody

• Catalog Number: orb1527755
• Category: Antibodies
• Description: ZCCHC8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-ZCCHC8 antibody A301-806A.; IP - 2 - 5 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:1,000 - 1:5,000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ZCCHC8
• UniProt ID: Q6NZY4
• NCBI: NP_060082.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: TRAMP-like complex RNA-binding factor ZCCHC8; zinc
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ZCCHC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ZCCHC8 antibody orb1527755 used for IP at 3 µg/mg lysate.

Detection of human ZCCHC8 by immunohistochemistry.Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZCCHC8 (Cat. No. orb1527755) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 2, 000 (0.5µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.