ZBTB46 Antibody / BTBD4 / ZNF340

• Catalog Number: orb2636867
• Category: Antibodies
• Description: BTBD4 (BTB/POZ domain-containing protein 4), also known as zinc finger protein 340 (ZNF340) or zinc finger and BTB domain-containing protein 46 (ZBTB46), is a 589 amino acid protein that contains one BTB/POZ domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ inter- actions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this tran- scriptional repression is attenuated. This suggests that BTBD4 may play a role in transcription regulation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-ZBTB46-2B8
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human Zinc finger and BTB domain-containing protein 46 protein was used as the immunogen for the ZBTB46 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ZBTB46 antibody is available for research use only.
• UniProt ID: Q86UZ6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ZBTB46 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

FACS staining of PFA-fixed human HeLa cells with ZBTB46 antibody (blue, clone PCRP-ZBTB46-2B8), and unstained cells (red).

IHC staining of FFPE human tonsil tissue with ZBTB46 antibody (clone PCRP-ZBTB46-2B8). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZBTB46 antibody (clone PCRP-ZBTB46-2B8). These results demonstrate the foremost specificity of the PCRP-ZBTB46-2B8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free ZBTB46 antibody (PCRP-ZBTB46-2B8) as confirmation of integrity and purity.