ZBTB40 Antibody

• Catalog Number: orb1527532
• Category: Antibodies
• Description: ZBTB40 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 1140 and 1190
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ZBTB40
• UniProt ID: Q9NUA8
• NCBI: NP_055685.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: zinc finger and BTB domain-containing protein 40;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ZBTB40 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 µg for WB) and HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-ZBTB40 antibody orb1527532 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. ZBTB40 was also immunoprecipitated by rabbit anti-ZBTB40 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Detection of human ZBTB40 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ZBTB40 (Cat. No. orb1527532) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB