ZAP70, Phospho (Y493/Y494) Antibody

• Catalog Number: orb1527393
• Category: Antibodies
• Description: ZAP70, Phospho (Y493/Y494) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP
• Reactivity: Human
• Immunogen: surrounding tyrosine 493/494
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. orb1527393 has not performed satisfactorily when used for WB of phospho ZAP70 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ZAP70.; IP - 2 - 5 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ZAP70, Phospho (Y493/Y494)
• UniProt ID: P43403
• NCBI: NP_001070.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ZAP-70; syk-related tyrosine kinase; zeta chain of
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of Phosphorylation of human ZAP70 on Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody was used at 3 µg/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated.

Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies used for IP at 3 µg/mg lysate.