ZAP3 Antibody

• Catalog Number: orb1523718
• Category: Antibodies
• Description: ZAP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 1350 and 1400
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ZAP3
• UniProt ID: P49750
• NCBI: NP_062535.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ZAP113; ZAP3; YLP motif-containing protein 1; prot
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ZAP3 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-ZAP3 antibody orb1523718 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ZAP3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ZAP3 antibody orb1523718 used for IP at 6 µg/mg lysate.

Detection of human ZAP3 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZAP3 (Cat. No. orb1523718) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB