YWHAB Antibody

• Catalog Number: orb1262717
• Category: Antibodies
• Description: YWHAB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Monkey, Mouse, Rat, Zebrafish
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human YWHAB.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 28 kDa
• Target: YWHAB
• UniProt ID: P31946
• NCBI: P31946
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: 14-3-3 protein beta/alpha, Protein 1054, Protein k
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Antibody is used in Western blot to detect YWHAB in Jurkat cell lysate.

Western blot analysis of YWHAB using rabbit polyclonal YWHAB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene.

Confocal immunofluorescent analysis of YWHAB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).