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Catalog Number | orb259643 |
---|---|
Category | Antibodies |
Description | Yes1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Yes1 (7-35aa KENKSPAIKYRPENTPEPVSTSVSHYGAE), different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 60801 MW |
UniProt ID | P07947 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Tyrosine-protein kinase Yes;2.7.10.2;Proto-oncogen Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Yes1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of YES1 using anti-YES1 antibody.Lane 1:human U20S cell; 2:human CACO-2 cell; 3:human A431 cell; 4:human HepG2 cell; 5:human MCF-7 cell; 6:human PC-3 cell.
IHC analysis of YES1 using anti-YES1 antibody. YES1 was detected in a paraffin-embedded section of human mammary cancer tissue.
DOT, IHC-P | |
Mouse, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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