YBX3 Antibody / Y-box-binding protein 3

• Catalog Number: orb1825867
• Category: Antibodies
• Description: Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Apr 2022]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-YBX3-2D12
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Y-box-binding protein 3 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Y-box-binding protein 3 antibody is available for research use only.
• UniProt ID: P16989
• Storage: Aliquot the Y-box-binding protein 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of PFA-fixed human HeLa cells with Y-box-binding protein 3 antibody (clone PCRP-YBX3-2D12) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Immunofluorescent staining of PFA-fixed human HeLa cells with Y-box-binding protein 3 antibody (clone PCRP-YBX3-2D12) followed by goat anti-mouse IgG-CF488 (green); Red = CF640R phalloidin.

IHC staining of FFPE human bladder carcinoma with Y-box-binding protein 3 antibody (clone PCRP-YBX3-2D12). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using Y-box-binding protein 3 antibody (clone PCRP-YBX3-2D12). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.