YB1/YBX1 Antibody

• Catalog Number: orb259642
• Category: Antibodies
• Description: YB1/YBX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human YB1 (293-320aa ENPKPQDGKETKAADPPAENSSAPEAEQ), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35924 MW
• UniProt ID: P67809
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nuclease-sensitive element-binding protein 1;CCAAT
• Note: For research use only
• Application notes: WB: The detection limit for YB1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-YB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of YB1 using anti-YB1 antibody.Lane 1:human HeLa cell; 2:human MCF-7 cell; 3:human Jurkat cell; 4:human T47D cell; 5:rat spleen tissue; 6:mouse RAW264.7 cell.

IF analysis of YB1 using anti-YB1 antibody. YB1 was detected in immunocytochemical section of A431 cells.

IHC analysis of YB using anti-YB antibody.YB was detected in frozen section of human placenta tissue.

IHC analysis of YB using anti-YB antibody.YB was detected in paraffin-embedded section of mouse testis tissue.

IHC analysis of YB using anti-YB antibody.YB was detected in paraffin-embedded section of rat testis tissue.

IHC analysis of YB using anti-YB antibody.YB was detected in paraffin-embedded section of human lung cancer tissue.