XPNPEP1 Antibody [SAIC-07E-1], Rabbit IgG

• Catalog Number: orb1672073
• Category: Antibodies
• Description: XPNPEP1 Antibody [SAIC-07E-1], Rabbit IgG
• Clonality: Recombinant
• Clone Number: SAIC-07E-1
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Peptide "TLSLDEVYLIDSGAQYK" derived from Xaa-Pro aminopeptidase 1 conjugated to KLH.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: XPNPEP1
• UniProt ID: Q9NQW7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Xaa-Pro aminopeptidase 1, XPNPEPL, XPNPEPL1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of HeLa cells with anti-XPNPEP1 (SAIC-07E-1). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-07E-1 (orb1672073) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672073- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-XPNPEP1 antibody SAIC-07E-1. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-07E-1 (orb1672073 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot using anti-XPNPEP1 antibody SAIC-07E-1. HeLa(A) (0.003 ug/ml)- Jurkat(B) (0.001 ug/ml)- MCF7(C) (0.001 ug/ml)- HepG2(D) (0.003 ug/ml)- A549(E) (0.001 ug/ml) and HEK293(F) (0.003 ug/ml) cell lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-07E-1 (orb1672073)- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.