XCR1 Antibody

• Catalog Number: orb1906381
• Category: Antibodies
• Description: XCR1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MARX10
• Tested applications: FC, IHC, In vivo
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 × 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: XCR1
• UniProt ID: Q9R0M1
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: mXCR1; Ccxcr1; Chemokine XC receptor 1; Lymphotact
• Note: For research use only
• Application notes: This antibody was used to stain cryostat sections (12 μm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20% of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012; PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1+ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay; MARX10-OVA was found to be highly effective in inducing CD8+ T cell cytotoxicity in vivo (Hartung et al., 2015; PMID: 25520399).
• Expiration Date: 12 months from date of receipt.