WWP1 monoclonal antibody (M01), clone 1A7

• Catalog Number: orb2291454
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant WWP1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1A7
• Tested applications: ELISA, IF, IHC-P, IP, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: WWP1 (NP_008944, 152 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: CSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGT
• NCBI: NP_008944
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged WWP1 is 0.03 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

Immunoprecipitation of WWP1 transfected lysate using anti-WWP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WWP1 MaxPab rabbit polyclonal antibody.

Western Blot analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody (M01), clone 1A7. Lane 1: WWP1 transfected lysate(105.2 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (37.73 KDa).