WWOX Antibody (monoclonal, 3D10)

• Catalog Number: orb527044
• Category: Antibodies
• Description: WWOX Antibody (monoclonal, 3D10)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D10
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: E. coli-derived human WWOX recombinant protein (Position: M1-D245).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 47 kDa
• UniProt ID: Q9NZC7
• Sensitivity: < 10pg/ml
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: WW domain-containing oxidoreductase; Fragile site
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-WWOX antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB:1:human placenta tissue;2:T-47D;3:HepG2;4:U-87MG;5:A549;6:HeLa;7:PC-3 cell;8:Caco-2 cell;9:rat testicular tissue;10:rat brain tissue;11:rat liver tissue;12:mouse testicular tissue;13:mouse brain tissue;14:mouse liver tissue;15:NIH/3T3 cell.

IF analysis of WWOX using anti-WWOX antibody. WWOX was detected in immunocytochemical section of A431 cells.

IHC analysis of WWOX using anti-WWOX antibody. WWOX was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of WWOX using anti-WWOX antibody. WWOX was detected in paraffin-embedded section of human ovarian cancer tissue.

IHC analysis of WWOX using anti-WWOX antibody. WWOX was detected in paraffin-embedded section of rat testis tissue.

IHC analysis of WWOX using anti-WWOX antibody. WWOX was detected in paraffin-embedded section of mouse testis tissue.