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Catalog Number | orb1274254 |
---|---|
Category | Antibodies |
Description | WT1 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | RMWT1-1 |
Tested applications | IF, IHC-P |
Reactivity | Human |
Isotype | IgG, k |
Immunogen | A recombinant protein was used as the immunogen for the WT1 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Immunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the WT1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | WT1 |
UniProt ID | P19544 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | WT1, GUD, WAGR, WIT-2, WT33, AWT1, NPHS4, EWS-WT1 Read more... |
Note | For research use only |
Application notes | Immunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the WT1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human kidney stained with WT1 antibody (clone RMWT1-1).
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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