WT1 Antibody

• Catalog Number: orb1274231
• Category: Antibodies
• Description: WT1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: WT1/1434R
• Tested applications: FC, IF, IHC-P
• Reactivity: Human, Rat
• Isotype: IgG, k
• Immunogen: A recombinant protein was used as the immunogen for the recombinant WT1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: WT1
• UniProt ID: P19544
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: WT1, GUD, WAGR, WIT-2, WT33, AWT1, NPHS4, EWS-WT1
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10e6 cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human mesothelioma tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

IHC testing of FFPE rat testis tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant WT1 antibody (clone WT1/1434R) as confirmation of integrity and purity.