WT1 Antibody

• Catalog Number: orb1252004
• Category: Antibodies
• Description: WT1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: WLMT1-1
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A recombinant human protein was used as the immunogen for the WT1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the WT1 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: WT1
• UniProt ID: P19544
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: WT1, GUD, WAGR, WIT-2, WT33, AWT1, NPHS4, EWS-WT1
• Note: For research use only
• Application notes: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Titering of the WT1 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human kidney stained with WT1 antibody (WT1/857).