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Catalog Number | orb259639 |
---|---|
Category | Antibodies |
Description | Wnt2b Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt2b (87-112 aa QRYPDIMRSVGEGAREWIRECQHQFR), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 43770 MW |
UniProt ID | Q93097 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein Wnt-2b;Protein Wnt-13;WNT2B;WNT13; Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Wnt2b is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of WNT2B using anti-WNT2B antibody.Lane 1:22RV1 Cell.
IHC analysis of WNT2B using anti-WNT2B antibody. WNT2B was detected in a paraffin-embedded section of mouse brain tissue.
IHC analysis of WNT2B using anti-WNT2B antibody. WNT2B was detected in a paraffin-embedded section of rat brain tissue.
IHC analysis of WNT2B using anti-WNT2B antibody. WNT2B was detected in a paraffin-embedded section of human lung cancer tissue.
ELISA, IHC, WB | |
Canine, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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