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    WNT10B antibody

    Catalog Number: orb668652

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb668652
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to WNT10B
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant fusion protein of human WNT10B. The exact sequence is proprietary.
    Dilution rangeWB: 1-500-2000
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetWNT10B
    Entrez22410, 7480
    UniProt IDP48614, O00744
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesAnti-WNT12 antibody, anti-Protein Wnt-10b antibody
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    WNT10B antibody

    Western blot analysis of WNT10B expression in HeLa (A) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)

    WNT10B antibody

    Immunohistochemical analysis of WNT10B staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    WNT10B antibody

    Immunofluorescent analysis of WNT10B staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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      ELISA,  WB

      Human, Mouse

      Rabbit

      Polyclonal

      Unconjugated

      50 μl, 100 μl
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