WNT10B Antibody (Center)

• Catalog Number: orb1929952
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19661
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 43000 Da
• Target: This WNT10B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from the Central region of human WNT10B.
• UniProt ID: O00744
• NCBI: NP_003385.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein Wnt-10b, Protein Wnt-12, WNT10B, WNT12
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of MDA-231 cells using WNT10B Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

WNT10B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT10B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-WNT10B Antibody (Center) at 1:2000 dilution. Lane 1: Rat skeletal muscle whole lysate. Lane 2: Human skeletal muscle whole lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.