Wnt1 antibody

• Catalog Number: orb344437
• Category: Antibodies
• Description: Wnt1 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 13F9
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: Synthetic peptide corresponding to an internal region of human Wnt1 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:20,000, WB: 1:175 - 1:250
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Wnt1 antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P04628
• NCBI: 139743
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-WNT1 antibody, INT1, Proto-oncogene pro
• Note: For research use only
• Application notes: This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse testis lysate using Wnt1 antibody