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Wnt1 Antibody
SKU: orb344437
Description
Images & Validation
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| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:4,000 - 1:20,000, WB: 1:175 - 1:250 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 13F9 |
| Immunogen | Synthetic peptide corresponding to an internal region of human Wnt1 protein. |
| Purity | Anti-Wnt1 antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above. This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined. |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-WNT1 antibody, INT1, Proto-oncogene protein Wnt-1
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Western blot using Biorbyt's Protein A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kDa Wnt1 protein. Primary antibody was used at a 1:500 dilution.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Wnt1 Antibody (orb344437)
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