WIZ Antibody

• Catalog Number: orb1520290
• Category: Antibodies
• Description: WIZ Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: between 1601 and 1651
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 20-40 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: WIZ
• UniProt ID: O95785
• NCBI: O95785.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: protein Wiz; ZNF803; zinc finger protein 803; wide
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse WIZ by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-WIZ antibody (orb1520290) used for WB at 0.1 mg/ml. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human WIZ by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-WIZ antibody (orb1520290) used for IP at 2 µg per reaction.