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WEE1 Rabbit Polyclonal Antibody
SKU: orb499671
Featured
Description
Research Area
CDK Regulators, Cell Cycle
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human WEE1 (151-250/646aa) |
| Target | WEE1 |
| Molecular Weight | 72 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DKFZp686I18166; EC 2.7.10.2; FLJ16446; Wee 1; WEE-1; WEE1 protein; Wee1 homolog; WEE1 homolog S. pombe; Wee1 like protein kinase; Wee1 tyrosine kinase; Wee1+homolog; Wee1+S. pombe homolog; WEE1A; Wee1A kinase; WEE1hu; WEE1 tyrosine kinase isoform 1; MGC105683; WEE1_HUMAN.
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Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (WEE1) Polyclonal Antibody, Unconjugated (orb499671) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Heart (Rat) Lysate at 40 ug, Primary: Anti-WEE1 (orb499671) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72 kD, Observed band size: 72 kD.
Quick Database Links
Gene Symbol
WEE1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
WEE1 Rabbit Polyclonal Antibody (orb499671)
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