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Catalog Number | orb259636 |
---|---|
Category | Antibodies |
Description | WASP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human WASP (129-156aa ADEDEAQAFRALVQEKIQKRNQRQSGDR), different from the related mouse sequence by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunocytochemistry , 0.5-1μg/ml, Human, -Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 52913 MW |
UniProt ID | P42768 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Wiskott-Aldrich syndrome protein;WASp;WAS;IMD2; Read more... |
Note | For research use only |
Application notes | WB: The detection limit for WASP is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of WASP using anti-WASP antibody.Lane 1:Rat Liver tissue;2:Human Placenta tissue;3:Rat Spleen tissue;4:Rat Pancreas tissue;5:HEPG2 cell;6:HELA cell;7;HEPA cell;8;22RV1 Cell.
IHC analysis of WAS using anti-WAS antibody.WAS was detected in frozen section of human placenta tissue.
IHC analysis of WAS using anti-WAS antibody.WAS was detected in immunocytochemical section of a549 cell.
IHC analysis of WAS using anti-WAS antibody.WAS was detected in paraffin-embedded section of human lung cancer tissue.
ELISA, FC, IF, IHC | |
Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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