VTCN1 Antibody / B7H4

• Catalog Number: orb2642354
• Category: Antibodies
• Description: T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: B7H4/2652R
• Tested applications: ELISA, FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant human partial protein was used as the immunogen for this recombinant B7-H4 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant B7-H4 antibody is available for research use only.
• UniProt ID: Q7Z7D3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant H7-B4 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human testicular carcinoma with recombinant B7-H4 antibody (clone B7H4/2652R). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant B7-H4 antibody (clone B7H4/2652R) as confirmation of integrity and purity.

Immunofluorescent staining of permeabilized human SKBR-3 cells with recombinant B7-H4 antibody (clone B7H4/2652R, green) and Reddot nuclear stain (red).

Flow cytometry testing of permeabilized human SKBR-3 cells with recombinant B7-H4 antibody (clone B7H4/2652R); Red = isotype control, Blue = recombinant B7-H4 antibody.