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Catalog Number | orb1231330 |
---|---|
Category | Proteins |
Description | VSIG3 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Fc Tag |
Dilution range | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.7 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 49.7 kDa |
Target | IGSF11 |
UniProt ID | Q5DX21 |
Protein Sequence | Leu 23 - Gly 241 |
Source | HEK293 cells |
NCBI | NP_001015887.1 |
Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | VSIG3,IgSF11,CXADRL1,Bt-IgSF,CT119 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.7 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human VSIG3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human VSIG3, Fc Tag at 5 ug/ml (100 uL/well) can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.313-10 ug/ml.
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 49.4 kDa after removal of the signal peptide. The apparent molecular mass of IGSF11-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
43.7 kDa | |
E.Coli |
Unconjugated | |
95% | |
49.7 kDa | |
Human VSIG3, Fc Tag (orb612144) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Gly 241 (Accession # Q5DX21-1). |
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