VPS25 Antibody

• Catalog Number: orb19138
• Category: Antibodies
• Description: Goat polyclonal antibody to VPS25
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• Target: VPS25
• Protein Sequence: QPNVDTRQKQ
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: VPS25; vacuolar protein sorting 25 (yeast); HGNC:2
• Note: For research use only
• Application notes: WB: Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Kidney. Recommended concentration: 6-8µg/ml.
• Expiration Date: 12 months from date of receipt.

orb19138 (0.5ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19138 (10ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.