VPS11 Antibody

• Catalog Number: orb1246796
• Category: Antibodies
• Description: VPS11 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HALQEKDTQTKLE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: VPS11
• UniProt ID: Q9H270
• NCBI: NP_068375.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: VPS11, vacuolar protein sorting 11 (yeast) , HGNC:
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246796 (3.8 ug/ml) staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246796 (4 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.