Von Willebrand Factor/VWF Antibody

• Catalog Number: orb182394
• Category: Antibodies
• Description: Von Willebrand Factor/VWF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC, IHC-Fr, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 309265 MW
• UniProt ID: P04275
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: von Willebrand factor;vWF;von Willebrand antigen 2
• Note: For research use only
• Application notes: WB: The detection limit for VWF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-VWF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of VWF using anti-VWF antibody.Lane 1:HT1080 Cell.

IHC analysis of VWF using anti-VWF antibody.VWF was detected in paraffin-embedded section of Human Lung Cancer Tissue.

IHC analysis of VWF using anti-VWF antibody.VWF was detected in frozen section of human placenta tissue.