Vitronectin/VTN Antibody

• Catalog Number: orb259635
• Category: Antibodies
• Description: Vitronectin/VTN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Vitronectin (446-472 aa RVNLRTRRVDTVDPPYPRSIAQYWLGC), different from the related mouse sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 54306 MW
• UniProt ID: P04004
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Vitronectin;VN;S-protein;Serum-spreading factor;V7
• Note: For research use only
• Application notes: WB: The detection limit for Vitronectin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Vitronectin using anti-Vitronectin antibody.Lane 1:Rat Liver Tissue;2:Rat Lung Tissue;3:HEPG2 Cell;4:HELA Cell;5:HEPA Cell.

IHC analysis of Vitronectin using anti-Vitronectin antibody. Vitronectin was detected in a paraffin-embedded section of human lung cancer tissue.