Vitamin D3 Receptor (phospho-S208) antibody

• Catalog Number: orb304658
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Vitamin D3 Receptor
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Primate
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S208 of human VDR protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: VDR
• Entrez: 24873, 22337, 7421
• UniProt ID: P13053, P11473, P48281
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-VDR antibody, anti-VDR_HUMAN antibody, anti-V
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of VDR (Phospho-S208) expression in HCT116 (A), HEK293T (B) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 43 kD)

Immunofluorescent analysis of VDR (Phospho-S208) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).