Vitamin D Binding protein Mouse Monoclonal Antibody

SKU: orb1606324

Description

Vitamin D Binding protein Mouse Monoclonal Antibody

Research Area

Cancer Research, Hormone Biosynthesis, Vitamins and Minerals

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B7E3
• Immunogen: Recombinant human Vitamin D Binding protein (7-474/474aa)
• Target: GC
• Molecular Weight: 53 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DBP; DBP-maf; DBP/GC; GRD3; Gc-MAF; GcMAF; HEL-S-51; VDB; VDBG; VDBP; VTDB_HUMAN; GC; Gc protein-derived macrophage activating factor (Gc-MAF | GcMAF); Gc-globulin; Group-specific component (Gc); Vitamin D-binding protein-macrophage activating factor (DBP-maf);

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vitamin D Binding protein) Monoclonal Antibody, Unconjugated (orb1606324) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vitamin D Binding protein) Monoclonal Antibody, Unconjugated (orb1606324) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Vitamin D Binding protein) Monoclonal Antibody, Unconjugated (orb1606324) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human Plasma, Primary: Anti-Vitamin D Binding protein (orb1606324) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 51 kDa, Observed band size: 53 kDa.

Quick Database Links

Gene Symbol

GC

UniProt

P02774