VISTA Detection Set (Risk Free)

• Catalog Number: orb1227439
• Category: Assays and Kits
• Description: VISTA Detection Set (Risk Free)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, IgG2b
• Immunogen: VISTA antibodies were raised against the extracellular domain of human VISTA.
• Concentration: Antibody 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Antibodies can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PBS containing 0.02% sodium azide.
• Note: For research use only
• Expiration Date: Please enquire.

Western blot analysis of VISTA in over expressing HEK293 cells using orb1240290 antibody at (A) 0.25 μg/ml, (B) 0.5 μg/ml, and (C) 1 μg/ml.

Immunocytochemistry of VISTA in over expressing HEK293 cells using (A) orb1240290, (B) orb1240293, (C) orb1240295, (D) orb1240296, and (E) control mouse IgG antibody at 1 μg/ml.

Immunofluorescence of VISTA in over expressing HEK293 cells using (A) orb1240290, (B) orb1240293, (C) orb1240295, (D) orb1240296, and (E) control mouse IgG antibody at 2 μg/ml.

Immunofluorescence of VISTA in human spleen tissue using (A) orb1240290, (B) orb1240293, (C) orb1240295, (D) orb1240296, and (E) control mouse IgG antibody at 10 μg/ml.

Immunohistochemistry of VISTA in human lymphoma tissue using (A) orb1240290, (B) orb1240293, (C) orb1240295, (D) orb1240296, and (E) control mouse IgG antibody at 2 μg/ml.

Flow cytometry analysis of VISTA over expressing HEK293 cells using (A) orb1240290, (B) orb1240293, (C) orb1240295, (D) orb1240296, and (E) control mouse IgG antibody at 10 μg/ml. Blue: untransfected HEK293 cells. Yellow: VISTA over expressing HEK293 cells.