Visfatin/NAMPT Antibody

• Catalog Number: orb371743
• Category: Antibodies
• Description: Visfatin/NAMPT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Visfatin recombinant protein (Position: L62-N337). Human Visfatin shares 97.1% and 96.7% amino acid (aa) sequence identity with mouse and rat Visfatin, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA , 0.1-0.5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 55521 MW
• UniProt ID: P43490
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nicotinamide phosphoribosyltransferase;NAmPRTase;N
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Visfatin using anti-Visfatin antibody.Lane 1:human U87 cell;2:human A431 cell;3:human Daudi cell;4:human U20S cell;5:human K562 cell;6:human A549 cell;7:human HL-60 cell;8:mouse testis tissue;9:mouse RAW264.7 cell;10:rat brain tissue.

IHC analysis of Visfatin using anti-Visfatin antibody. Visfatin was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of Visfatin using anti-Visfatin antibody. Visfatin was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Visfatin using anti-Visfatin antibody. Visfatin was detected in a paraffin-embedded section of rat intestine tissue.