Vimentin (phospho-S56) antibody

• Catalog Number: orb1474480
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Vimentin (phospho-S56)
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S56 of human Vimentin protein. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: VIM
• Entrez: 7431
• UniProt ID: P08670
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: Vimentin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Vimentin (Phospho-S56) expression in U251MG (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 54 kD)

Immunofluorescent analysis of Vimentin (Phospho-S56) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.