Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody

• Catalog Number: orb2317883
• Category: Antibodies
• Description: Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VIL1/1314
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Western Blot (1-2ug/ml for 60 minutes at RT); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 93kDa
• Target: VIL1
• Entrez: 7429
• UniProt ID: P09327
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: VIL1; Villin-1; Villin1
• Note: For research use only
• Application notes: Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.
• Expiration Date: 12 months from date of receipt.