VGLUT1 antibody

• Catalog Number: orb149730
• Category: Antibodies
• Description: Mouse monoclonal to VGlut1 (RPE). VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S28-9
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: RPE
• MW: 52kDa
• Target: VGLUT1
• Entrez: 116638
• UniProt ID: Q62634
• NCBI: NP_446311.1
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: BNPI antibody, SLC17A1 antibody, SLC17A1/VGLUT1 an
• Note: For research use only
• Application notes: 1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.